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Book Summary:
We have developed a variety of functional genomics tools for the identification and characterization of trait conferring genes in bacteria. These include: a series of broad host range vectors for the creation and screening of large a representational genomic libraries, a system for transposon based insertional mutagenesis that can provide greater 105 mutants, and allow for the assessment of polar affects, as well as a genome-wide DNA micro-array method for identifying genes and genetic elements that affect interesting phenotypes. In particular, this tool has been used to identify and characterize genes involved in environmentally controlled biofilm development in E. coli. |
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